hypnotic effect of ocimum basilicum on pentobarbital-induced sleep in mice
نویسندگان
چکیده
background sleep disorders are accompanied by several complications, and currently used soporific drugs can induce unwanted effects such as psychomotor impairment, tolerance, amnesia, and rebound insomnia. results hae increased the duration of pentobarbital-induced sleep at doses of 25, 50, and 100 mg/kg (p < 0.001). the hypnotic effect of hae was comparable to that induced by diazepam. similarly, wf, eaf, and nbf at 50 mg/kg could increase sleep duration. the sleep latency was decreased by hae (p < 0.01 - p < 0.001) and nbf (p < 0.001), but not by wf and eaf. the ld50 value for hae was found to be 2.4 g/kg. hae had no effect on the viability of neuronal pc12 cells and l929 fibroblast cells. objectives the present study was carried out to investigate if ocimum basilicum has a sleep-prolonging effect. materials and methods this work was an experimental study on 72 mice which were randomly divided into 9 groups: saline (control); diazepam (3 mg/kg, positive control); hydro-alcoholic extract (hae) of ocimum basilicum (25, 50, or 100 mg/kg); ethyl acetate fraction (eaf, 50 mg/kg); n-butanol fraction (nbf, 50 mg/kg); water fraction (wf, 50 mg/kg); and saline containing 10% dmso (vehicle for eaf and nbf). all the test compounds were injected intraperitoneally (ip) 30 minutes before pentobarbital administration (30 mg/kg). duration and latency of pentobarbital-induced sleep were recorded. also, ld50 of hae was determined and the cytotoxicity of hae was tested on neural and fibroblast cells using the mtt assay. conclusions the present data demonstrated that ocimum basilicum potentiates sleeping behaviors without any cytotoxicity. the main component (s) responsible for the hypnotic effects of this plant is most likely a non-polar agent (s) which is found in nbf. isolation of the active constituents may yield a novel sedative drug.
منابع مشابه
Hypnotic Effect of Ocimum basilicum on Pentobarbital-Induced Sleep in Mice
BACKGROUND Sleep disorders are accompanied by several complications, and currently used soporific drugs can induce unwanted effects such as psychomotor impairment, tolerance, amnesia, and rebound insomnia. OBJECTIVES The present study was carried out to investigate if Ocimum basilicum has a sleep-prolonging effect. MATERIALS AND METHODS This work was an experimental study on 72 mice which w...
متن کاملhypnotic effect of nepeta glomerulosa on pentobarbital-induced sleep in mice
background insomnia or sleeplessness is a disorder characterized by a personal incapability to falling or staying asleep for a desirable period of time, therefore disturb in quality of life. objectives the present study was carried out to investigate the effect of hydro-alcoholic extract of nepeta glomerulosa and its fractions on sleep-prolonging. materials and methods the extract and its fract...
متن کاملEffect of Methanolic Leaf Extract of Ocimum basilicum L. on Benzene-Induced Hematotoxicity in Mice
The aim of the present study was to investigate the protective role of methanolic leaf extract of Ocimum basilicum L. against benzene-induced hematotoxicity in Swiss albino mice. GC analysis and subacute toxicity level of the extract were tested. Mice were randomly divided into three groups among which II and III were exposed to benzene vapour at a dose 300 ppm × 6 hr/day × 5 days/week for 2 we...
متن کاملthe effect of aqueous extract of garlic on formalin-induced pain in male rats
چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...
15 صفحه اولEffect of Ocimum basilicum hydro-alcoholic extract on oxidative damage of brain tissue following seizures induced by pentylenetetrazole in mice
Introduction: A relationship between epileptic seizures and brain tissue oxidative damage has been suggested. Ocimum basilicum (O. basilicum) has been shown to have beneficial effects including hypnotic and protective against tissue oxidative damage. The present study was designed to evaluate the effects of O. basilicum hydro-alcoholic extract on oxidative damage of brain tissue following s...
متن کاملPotentiating Effects of Lactuca sativa on Pentobarbital-Induced Sleep
Traditionally, Lactuca sativa (lettuce) has been recommended for its hypnotic property.The present study was planned to investigate sleep-prolonging effect of this plant. The hydroalcoholicextract (HAE) of lettuce and its water fraction (WF), ethyl acetate fraction (EAF),and n-butanol fraction (NBF) were administrated (IP) to mice 30 min before the pentobarbitalinjection. Moreover, both in-vivo...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian red crescent medical journalجلد ۱۸، شماره ۷، صفحات ۰-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023